magl gene family  (Medicago)

Journal: Plant Biotechnology Journal

Article Title: Deciphering the role of monoacylglycerol lipases ( MAGL ) under abiotic stress and lipid metabolism in soybean ( Glycine max L.)

doi: 10.1111/pbi.70088

Figure Lengend Snippet: Similarity clustering and collinear synteny of monoacylglycerol lipase ( MAGL ) genes identified in different plants. (a) The heatmap visualizes the similarity of MAGL genes evaluated based on the BLASTP search. The clustering was done by using Markov clustering implemented in Tribe‐MCL. The OrthoFinder method was applied to infer sub‐families only if they overlap for 95% or more. The red colour indicates a high degree of similarity and dark blue little to no similarity. (b) Colinear synteny between Glycine max and Glycine soja . The upper blocks in orange represent the G. max chromosomes and the lower green blocks represent the G. soja chromosomes. The grey lines indicate the co‐linearity in the whole genome and red lines show co‐linearity among monoacylglycerol lipase (MAGL) genes.

Article Snippet: The tandem duplication was observed only in two MAGL genes in medicago.

Techniques:

Journal: Plant Biotechnology Journal

Article Title: Deciphering the role of monoacylglycerol lipases ( MAGL ) under abiotic stress and lipid metabolism in soybean ( Glycine max L.)

doi: 10.1111/pbi.70088

Figure Lengend Snippet: Phylogenetic distribution, conserved amino acid, and motifs of monoacylglycerol lipase ( MAGL ) genes in different plants. (a) The phylogenetic analysis of MAGL genes identified in five species namely soybean (purple circle), Glycine soja (yellow circle), medicago (blue circle), Arabidopsis (red circle), and rice (green circle) showing five major groups and their subgroups. The MAGL genes in group IV with AXSXG and SXSXG motif is highlighted in red colour. (b) Conserved amino acids and motifs in monoacylglycerol lipase ( MAGL ) genes. The circular representation of the multiple sequence alignment (MSA) shows the level of conservation for each amino acid. The circle lines in the center represent the connected pairs of positions with mutual information (MI) greater than 6.5. The information of each circle from outer to inner is the following: labels in the first (outer) circle indicate the alignment position and the amino acid code of the reference sequence. The coloured square boxes of the second circle indicate the MSA position conservation (highly conserved positions are in red, while less conserved ones are in blue). The third and fourth circles show the Proximity MI (pMI) and Cumulative MI (cMI) as histograms, facing inwards and outwards respectively.

Article Snippet: The tandem duplication was observed only in two MAGL genes in medicago.

Techniques: Sequencing

Journal: Plant Biotechnology Journal

Article Title: Deciphering the role of monoacylglycerol lipases ( MAGL ) under abiotic stress and lipid metabolism in soybean ( Glycine max L.)

doi: 10.1111/pbi.70088

Figure Lengend Snippet: Expression of soybean monoacylglycerol lipases ( MAGL ) genes. (a) The heatmap showing the expression of MAGL in different tissues of soybean, and under drought and heat stress conditions. The expression data were obtained from the available transcriptomic studies. (b) Expression of soybean monoacylglycerol lipases ( MAGL ) genes under silicon and Arsenic supplementation. The expression of MAGL genes in root, stem, and leaf tissues of soybean plants under silicon (Si) and arsenic (As(III)) treatment. The expression was evaluated using quantitative real‐time PCR (qRT‐PCR).

Article Snippet: The tandem duplication was observed only in two MAGL genes in medicago.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Plant Biotechnology Journal

Article Title: Deciphering the role of monoacylglycerol lipases ( MAGL ) under abiotic stress and lipid metabolism in soybean ( Glycine max L.)

doi: 10.1111/pbi.70088

Figure Lengend Snippet: (A) Visualization of lipid bodies using BODIPY493/503493/503 dye in yeast cells carrying empty pYES2.1 plasmids (Control) and yeast cells overexpressing Glyma.13G183800. (B) Histogram of BODIPY493/503 inflorescence in control versus MAGL overexpression yeast transformants. The Y axis shows the number of cells and the X axis shows fluorescence intensity (BODIPY493/503). (a) BODIPY493/503 inflorescence in control and Glyma.13G183800 gene overexpression yeast transformant (b) BODIPY493/503 inflorescence in control and Glyma.05G248100 gene overexpression yeast transformant (c) BODIPY493/503 inflorescence in control and Glyma.09G184000 gene overexpression yeast transformant (d) BODIPY493/503 inflorescence in control and Gm10G273600 gene overexpression yeast transformant. (C) Quantification of inflorescence of BODIPY 493/503 stained yeast lipid bodies using flow cytometry. The X‐axis represents fluorescence intensity whereas the Y‐axis represents the number of stained cells. Decreasing fluorescence intensity depicts diminishing lipid bodies in yeast cells. (D) Yeast survival assay showing growth of yeast expressing four soybean MAGL genes on arsenic (III), manitol, and NaCl. Galactose was used to induce the MAGL gene expression.

Article Snippet: The tandem duplication was observed only in two MAGL genes in medicago.

Techniques: Control, Over Expression, Fluorescence, Staining, Flow Cytometry, Clonogenic Cell Survival Assay, Expressing, Gene Expression